Regulatory

Part:BBa_K1330002

Designed by: Kristian Jensen   Group: iGEM14_DTU-Denmark   (2014-09-30)

Constitutive promoter (J23105)

This BioBrick is the constitutive promoter BBa_J23105 in backbone pSB1C3. The BioBrick is flanked by the BioBrick prefix and suffix and is thus ready for being used in standard assembly.

Usage and Biology

This promoter is part of the Anderson promoter library, which is a collection of constitutive promoters of different strength, for use in E. coli. For more info on the Anderson promoter library and the characterization of it, see this page. The relative strength of this promoter has been measured to 0.24 relative to BBa_J23100, by Chris Anderson and the 2006 Berkeley iGEM team.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//rnap/prokaryote/ecoli/sigma70
//direction/forward
//chassis/prokaryote/ecoli
//promoter/Anderson
//regulation/constitutive
Parameters
None